"Hey Tennessee kid, I'm being quiet because you're in the next room. It's your last show, it's the last Man of the Woods show tonight. I would say that I'm so proud of you but I feel like that's the understatement of the century and that somehow that I take ownership to what you've done this past year-and-a-half," Biel, 37, said.
"I'm just in absolute awe of you every night to see you up there in your glory doing what you've been put on this Earth to do," continued Biel, who proudly wore a Man of the Woods tour hat.
"You inspire me, you inspire Silas," the mother of one added about their 4-year-old son.
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"You inspire everyone around you. All of your fans, all of your friends and family to work as hard and with so much passion as you do. You are truly a sight to behold," Biel said. "I'm so happy that you're going to get some time off. I'm so happy for you and for us."
Biel, who held back tears towards the end of her message, wrapped up her selfie video with a heartfelt congratulations.
"Also I'm so sad that I won't be able to see you up there again for a while," she said. "You make my heart burst with joy and love when I see you doing your thing baby. I'm your No. 1 fan, I love you."
Timberlake responded to his wife's video, writing in the comments section: "I mean…I'm not crying. You're CRYING! I can't with this message. I love you so much."
Timberlake, 37, kicked off his international Man of the Woods Tour on March 13, 2018, in Toronto. In June, he hopped across the pond for the European leg, then returned to the U.S. for another North American leg in the fall, even adding a slew of new dates in Pennsylvania, New York, and Connecticut.
Timberlake was plagued by vocal issues throughout the global trek. In October 2018, the New York Times best-selling author of Hindsight announced on Instagram just hours before a show in New York City at Madison Square Garden that his doctors had ordered him to rest.
The *NSYNC alum previously had an operation to remove his throat nodules—benign growths on the vocal cords—in 2005.